ethyl 3- (4- (methylamino) -3-nitro-N- (pyridin-2-yl) benzamido) propanoate (CAS # 429659-01-8)
Ọrọ Iṣaaju
Ethyl 3- (4- (methylamino) -3-nitro-N- (pyridin-2-yl) benzoylamido) propionate, ti a tun mọ ni MTT (Meta-Toluidine ortho-Toluenesulphonic acid triazine ester), jẹ nkan ti kemikali. O ni awọn ohun-ini wọnyi:
Irisi: White ri to
Solubility: Tiotuka ninu awọn ọti, awọn ketones ati chloroform
Awọn lilo akọkọ ti MTT jẹ bi atẹle:
Iwadi nipa isedale: MTT ni lilo pupọ lati pinnu ilọsiwaju sẹẹli ati ṣiṣeeṣe sẹẹli. O ṣe iyipada MTT sinu ọja buluu ti o tuka ninu awọn sẹẹli nipa idinku rẹ, eyiti o le ṣee lo lati pinnu ṣiṣeeṣe sẹẹli.
Ni gbogbogbo, ọna igbaradi ti MTT le ṣee ṣe nipasẹ awọn igbesẹ wọnyi:
Methylaniline ni a ṣe pẹlu 2-nitrobenzoyl kiloraidi lati gba 3- (4-methylaminobenzoyl) benzoyl kiloraidi.
Ọja ti a gba ni igbesẹ 1 ni a ṣe pẹlu 2-pyridone lati ṣe ipilẹṣẹ 3- (4-methylaminobenzoylamido) benzoyl chloride.
Ọja ti a gba ni igbese 2 ni a ṣe pẹlu ethyl 3-aminopropionate lati ṣe ipilẹṣẹ ethyl 3- (4-methylaminobenzoylamido) propionate.
Ọja ikẹhin, MTT, jẹ mimọ nipasẹ crystallization tabi awọn ọna miiran.
Alaye Aabo: MTT jẹ nkan oloro, ati pe o yẹ ki o ṣe itọju lati lo ati sọ ọ daradara. Awọn ohun elo aabo ti o yẹ gẹgẹbi awọn ibọwọ, awọn gilaasi, ati awọn aṣọ ile-iyẹwu nilo lati wọ lakoko iṣẹ. Yago fun ifasimu, ifarakan ara, ati jijẹ. Nigbati o ba nlo tabi sisọnu egbin, tẹle awọn ilana ṣiṣe ailewu ti o yẹ, sọsọsọsọ ati nu eyikeyi ifihan airotẹlẹ. Ni ọran ti ifasimu eyikeyi tabi olubasọrọ ara, wa itọju ilera lẹsẹkẹsẹ.
