Fmoc-L-2-Aminobutyric acid (CAS# 135112-27-5)
Ewu ati Aabo
| Apejuwe Abo | S22 - Maṣe simi eruku. S24/25 - Yago fun olubasọrọ pẹlu awọ ara ati oju. |
| WGK Germany | 3 |
| HS koodu | 2924 29 70 |
| Kíláàsì ewu | IKANU |
Fmoc-L-2-Aminobutyric acid (CAS# 135112-27-5 )Ifihan
L-2- (fluorenylmethoxycarbonylamino) acid butyric, ti a tun mọ ni Fmoc-glutamic acid (L-2- (9-fluorenylmethoxycarbonylamino) butyric acid), jẹ nkan ti o ni nkan ti ara. Atẹle ni apejuwe diẹ ninu awọn ohun-ini rẹ, awọn lilo, awọn ọna ati alaye aabo:
Iseda:
-Irisi: Alailowaya tabi die-die ofeefee crystalline lulú;
-Molecular agbekalẹ: C26H23NO4;
-Molecular iwuwo: 413.47g / mol;
-yo ojuami: nipa 166-168 ℃;
-Solubility: Fmoc-glutamic acid jẹ tiotuka ni awọn ohun elo ti o wa ni erupẹ bi dimethylformamide ati hydrofluoric acid.
Lo:
Fmoc-glutamic acid, gẹgẹbi ẹgbẹ ẹgbẹ aabo, ni igbagbogbo lo ninu iṣelọpọ peptide, ni pataki ni iṣelọpọ ti awọn ilana peptide iduroṣinṣin ati iṣelọpọ ti awọn peptides Levenstein (Levenshtein peptide). O le ṣee lo bi agbedemeji sintetiki ti amino acids, tabi lo ninu iṣelọpọ alakoso ti o lagbara, kiromatofi omi iṣẹ ṣiṣe giga, ati bẹbẹ lọ.
Ọna Igbaradi:
Igbaradi ti Fmoc-glutamic acid ni gbogbogbo pẹlu awọn igbesẹ wọnyi:
1. Ninu eto anhydrous, L-glutamic acid ti ṣe atunṣe pẹlu Fmoc-OSu (fluorenylmethoxycarbonyl-chloro anhydride), ati iṣuu soda kiloraidi (NaCl) ni a lo lati ṣe igbelaruge ifarahan lati ṣe ina Fmoc-glutamic acid Fmoc-A iyọ.
2. Fun iyọ Fmoc-glutamic acid, o ti wa ni tituka ni epo-ara (gẹgẹbi dimethylformamide) ati ki o tẹriba si ọna isọdi ti o yẹ (gẹgẹbi crystallization) lati gba Fmoc-glutamic acid.
Alaye Abo:
Fmoc-glutamic acid jẹ iduroṣinṣin gbogbogbo ati majele kekere labẹ iṣẹ ṣiṣe deede. Ṣugbọn gẹgẹbi ohun elo Organic, tun nilo lati fiyesi si awọn ọna aabo wọnyi:
-Yẹra fun eruku ifasimu, olubasọrọ pẹlu awọ ara ati oju;
- Lakoko iṣẹ, wọ awọn ibọwọ aabo, awọn gilaasi ati aṣọ aabo ti o pade awọn ibeere aabo;
-Ṣiṣẹ ni agbegbe ti o ni afẹfẹ daradara lati yago fun ifihan taara fun igba pipẹ;
-Ti o ba jẹ pe a gbọdọ sọ egbin lati inu agbo-ara yii, o yẹ ki o sọnu daradara ni ibamu pẹlu awọn ilana agbegbe.
Iseda:
-Irisi: Alailowaya tabi die-die ofeefee crystalline lulú;
-Molecular agbekalẹ: C26H23NO4;
-Molecular iwuwo: 413.47g / mol;
-yo ojuami: nipa 166-168 ℃;
-Solubility: Fmoc-glutamic acid jẹ tiotuka ni awọn ohun elo ti o wa ni erupẹ bi dimethylformamide ati hydrofluoric acid.
Lo:
Fmoc-glutamic acid, gẹgẹbi ẹgbẹ ẹgbẹ aabo, ni igbagbogbo lo ninu iṣelọpọ peptide, ni pataki ni iṣelọpọ ti awọn ilana peptide iduroṣinṣin ati iṣelọpọ ti awọn peptides Levenstein (Levenshtein peptide). O le ṣee lo bi agbedemeji sintetiki ti amino acids, tabi lo ninu iṣelọpọ alakoso ti o lagbara, kiromatofi omi iṣẹ ṣiṣe giga, ati bẹbẹ lọ.
Ọna Igbaradi:
Igbaradi ti Fmoc-glutamic acid ni gbogbogbo pẹlu awọn igbesẹ wọnyi:
1. Ninu eto anhydrous, L-glutamic acid ti ṣe atunṣe pẹlu Fmoc-OSu (fluorenylmethoxycarbonyl-chloro anhydride), ati iṣuu soda kiloraidi (NaCl) ni a lo lati ṣe igbelaruge ifarahan lati ṣe ina Fmoc-glutamic acid Fmoc-A iyọ.
2. Fun iyọ Fmoc-glutamic acid, o ti wa ni tituka ni epo-ara (gẹgẹbi dimethylformamide) ati ki o tẹriba si ọna isọdi ti o yẹ (gẹgẹbi crystallization) lati gba Fmoc-glutamic acid.
Alaye Abo:
Fmoc-glutamic acid jẹ iduroṣinṣin gbogbogbo ati majele kekere labẹ iṣẹ ṣiṣe deede. Ṣugbọn gẹgẹbi ohun elo Organic, tun nilo lati fiyesi si awọn ọna aabo wọnyi:
-Yẹra fun eruku ifasimu, olubasọrọ pẹlu awọ ara ati oju;
- Lakoko iṣẹ, wọ awọn ibọwọ aabo, awọn gilaasi ati aṣọ aabo ti o pade awọn ibeere aabo;
-Ṣiṣẹ ni agbegbe ti o ni afẹfẹ daradara lati yago fun ifihan taara fun igba pipẹ;
-Ti o ba jẹ pe a gbọdọ sọ egbin lati inu agbo-ara yii, o yẹ ki o sọnu daradara ni ibamu pẹlu awọn ilana agbegbe.
Kọ ifiranṣẹ rẹ nibi ki o si fi si wa
