Isopropyl-beta-D-thiogalactopyranoside (CAS#367-93-1)
Ewu ati Aabo
| Awọn koodu ewu | R19 - Le dagba awọn ibẹjadi peroxides R40 - Ẹri to lopin ti ipa carcinogenic R66 - Ifarahan leralera le fa gbigbẹ ara tabi fifọ R36 / 37/38 - Irritating si oju, eto atẹgun ati awọ ara. |
| Apejuwe Abo | S23 – Maṣe simi oru. S24/25 - Yago fun olubasọrọ pẹlu awọ ara ati oju. S36 / 37 - Wọ aṣọ aabo ati awọn ibọwọ to dara. S22 - Maṣe simi eruku. S36 / 37/39 - Wọ aṣọ aabo to dara, awọn ibọwọ ati aabo oju / oju. S27 - Mu gbogbo aṣọ ti o ti doti kuro lẹsẹkẹsẹ. S26 - Ni ọran ti olubasọrọ pẹlu awọn oju, fi omi ṣan lẹsẹkẹsẹ pẹlu ọpọlọpọ omi ki o wa imọran iṣoogun. |
| WGK Germany | 3 |
| FLUKA BRAND F koodu | 10 |
| TSCA | Bẹẹni |
| HS koodu | 29389090 |
Ọrọ Iṣaaju
IPTG jẹ nkan ti nfa iṣẹ ṣiṣe ti β-galactosidase. Da lori abuda yii, nigbati DNA fekito ti pUC jara (tabi DNA fekito miiran pẹlu jiini lacZ) ti yipada pẹlu awọn sẹẹli piparẹ lacZ bi agbalejo, tabi nigbati DNA fekito ti M13 phage ti yipada, ti o ba ṣafikun X-gal ati IPTG. si alabọde awo, nitori α-complementarity ti β-galactosidase, ẹda recombinant le jẹ ni rọọrun yan ni ibamu si boya awọn ileto funfun (tabi plaques) han. Ni afikun, o tun le ṣee lo bi olupilẹṣẹ ikosile fun awọn ikosile ikosile pẹlu awọn olupolowo bii lac tabi tac. Tiotuka ninu omi, kẹmika, ethanol, tiotuka ni acetone, chloroform, insoluble in ether. O jẹ oludasilẹ ti β-galactosidase ati β-galactosidase. Ko ṣe hydrolyzed nipasẹ β-galactoside. O jẹ ojutu sobusitireti ti thiogalactosyltransferase. Agbekale: IPTG ti wa ni tituka ninu omi, ati ki o sterilized lati mura a ipamọ ojutu (0 · 1M). Ifojusi IPTG ikẹhin ni awo atọka yẹ ki o jẹ 0 · 2mM.
Kọ ifiranṣẹ rẹ nibi ki o si fi si wa
