N (alpha) -fmoc-N (epsilon)-(2-chloro-Z) -L-lysine (CAS# 133970-31-7)

Fmoc- (2-chlorobenzyloxycarbonyl) lysine jẹ itọsẹ amino acid ti o wọpọ pẹlu awọn ohun-ini wọnyi:1. Irisi: funfun okuta lulú;
2. agbekalẹ molikula: C26H24ClNO5;
3. Iwọn molikula: 459.92g / mol;
4. Solubility: Soluble ni Organic solvents bi dimethyl sulfoxide (DMSO), dimethyl formamide (DMF), dichloromethane, bbl, insoluble ninu omi;
5. Iyọkuro: nipa 170-175 ° C. Lilo akọkọ ti Fmoc- (2-chlorobenzyloxycarbonyl) lysine jẹ bi idaabobo ati ẹgbẹ ti n ṣiṣẹ ni iṣelọpọ ti polypeptides. Ẹgbẹ carboxyl rẹ le muu ṣiṣẹ lati ṣe agbekalẹ ester kan, eyiti lẹhinna faragba ifaseyin condensation pẹlu aloku amino acid lati ṣajọpọ pq polypeptide kan. Ẹgbẹ Fmoc le ni irọrun yọkuro lẹhin ifasẹyin ti pari lati fi han ohun elo amino ti o ni aabo.

Ọna ti ngbaradi Fmoc- (2-chlorobenzyloxycarbonyl) lysine ni gbogbogbo pẹlu awọn igbesẹ wọnyi:

1. fesi lysine pẹlu N-hydroxybutyrimide (Pbf) lati ṣafihan ẹgbẹ aabo kan;
2. ifasilẹ lysine-Pbf itọsẹ pẹlu 2-chlorobenzyl oti lati dagba Fmoc- (2-chlorobenzyloxycarbonyl) lysine;
3. A mu ọja naa jade pẹlu ohun elo ti o yẹ ati ti a sọ di mimọ nipasẹ crystallization lati gba ọja mimọ.

Nipa alaye ailewu, Fmoc- (2-chlorobenzyloxycarbonyl) lysine jẹ reagent kemikali ati pe o yẹ ki o mu awọn igbese ailewu ti o yẹ. Awọn ohun elo aabo ti o yẹ, gẹgẹbi awọn ibọwọ lab, awọn gilaasi, ati awọn aṣọ lab, yẹ ki o wọ lakoko idanwo naa. Yago fun ifasimu lulú tabi awọn ojutu, yago fun olubasọrọ pẹlu awọ ara ati oju. Ni ọran ti olubasọrọ lairotẹlẹ, fi omi ṣan lẹsẹkẹsẹ pẹlu ọpọlọpọ omi ki o wa iranlọwọ iṣoogun. Rii daju pe o ti lo ni agbegbe ile-iwadii ailewu ati fipamọ daradara lati yago fun awọn ijamba.